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KMID : 1094720210260061012
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Biotechnology and Bioprocess Engineering
2021 Volume.26 No. 6 p.1012 ~ p.1022
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Optimization and Modeling of Curdlan Production under Multi-physiological-parameters Process Control by Agrobacterium radiobacter Mutant A-15 at High Initial Glucose
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Yang Hui
Li Wenqiang
Chen Shuochang
Guo Xiaolei
Huang Fei
Zhu Ping
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Abstract
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The optimization and kinetic modeling of curdlan synthesis by Agrobacterium radiobacter mutant A-15 under multi-physiological-parameters process control, especially employing the on-line oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were performed in a 50-L fermentor. Results indicated that the maximum production of curdlan reach about 32.5 g/L with a glucose conversion yield of 49.5%, the maximum specific growth rate was about 0.5338 h?1, the kinetics equation were of positive significance for production. It also demonstrated that A-15 was insensitive to catabolite repression during polysaccharide production for its maintaining high conversion yield in the medium contained 6.6% (W/V) initial glucose. In addition, the OUR and CER at the stationary growth stage showed a positive correlation with curdlan synthesis. This work will provide more theoretical support for large-scale curdlan production.
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KEYWORD
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curdlan, optimization, OUR, CER, kinetic model
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